mini protein gel apparatus Search Results


sepharose  (GE Healthcare)
96
GE Healthcare sepharose
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page pre cast tris hcl gels  (Bio-Rad)
97
Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page pre cast tris hcl gels
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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sds page mini gel  (Bangalore Genei)
90
Bangalore Genei sds page mini gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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mini protean polyacrylamide gel  (Bio-Rad)
99
Bio-Rad mini protean polyacrylamide gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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mini protean tgx precast 1029 protein gel  (Bio-Rad)
99
Bio-Rad mini protean tgx precast 1029 protein gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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sds page gel electrophoresis  (Bio-Rad)
97
Bio-Rad sds page gel electrophoresis
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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criterion tgx stain free gels  (Bio-Rad)
96
Bio-Rad criterion tgx stain free gels
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
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bio rad mini protean gel  (Bio-Rad)
93
Bio-Rad bio rad mini protean gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
Bio Rad Mini Protean Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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separation gel  (Bio-Rad)
94
Bio-Rad separation gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
Separation Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tgx gel  (Bio-Rad)
99
Bio-Rad mini protean tgx gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
Mini Protean Tgx Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page tgx gel  (Bio-Rad)
96
Bio-Rad sds page tgx gel
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
Sds Page Tgx Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tetra cell blotting device  (Bio-Rad)
93
Bio-Rad mini protean tetra cell blotting device
LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a <t>formaldehyde-agarose</t> gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.
Mini Protean Tetra Cell Blotting Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a formaldehyde-agarose gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.

Journal: The Journal of Biological Chemistry

Article Title: Drosophila Low Temperature Viability Protein 1 (LTV1) Is Required for Ribosome Biogenesis and Cell Growth Downstream of Drosophila Myc (dMyc) *

doi: 10.1074/jbc.M114.607036

Figure Lengend Snippet: LTV1 is required for pre-rRNA processing. A, a schematic representation of Drosophila pre-rRNA processing. Pre-rRNA and its intermediates are designated pre, a, and d according to a previous report (32). External transcribed spacer (ETS), ITS1, and ITS2 are indicated. The 5ITS1 probe, which was used in hybridizing the 5′-region of ITS1, is indicated by black bar. B, total RNA was extracted from the second instar larvae of w1118, LTV1 mutant, and LTV1 mutant-expressing exogenous LTV1. Total RNA was separated in a formaldehyde-agarose gel. Pre-rRNA and its intermediates were examined by Northern blotting with the probe for 5ITS1. Each intermediate is indicated. An asterisk marks the 2.5-kb intermediate.

Article Snippet: 50 μl of protein A-Sepharose (GE Healthcare, 17-0974-04) was applied to the diluted lysates followed by incubating on a rotator for 30 min at 4 °C to remove proteins nonspecifically bound to Sepharose in the lysates.

Techniques: Mutagenesis, Expressing, Agarose Gel Electrophoresis, Northern Blot